Detecting topological variations of DNA at single-molecule level
نویسندگان
چکیده
منابع مشابه
DNA replication at the single-molecule level.
A cell can be thought of as a highly sophisticated micro factory: in a pool of billions of molecules - metabolites, structural proteins, enzymes, oligonucleotides - multi-subunit complexes assemble to perform a large number of basic cellular tasks, such as DNA replication, RNA/protein synthesis or intracellular transport. By purifying single components and using them to reconstitute molecular p...
متن کاملDetecting DNA damage at a single-molecule level by atomic force microscopy
DNA damage can be caused daily by as many as one million molecular lesions per cell by normal metabolic activities and environmental factors. The uncorrected lesions in critical genes (such as tumor suppressor genes) can inhibit a cell's ability to carry out its regular function and accordingly increase the possibility of tumor formation. Detecting DNA damage is the first step towards understan...
متن کاملProbing DNA-peptide interaction forces at the single-molecule level.
The versatility of chemical peptide synthesis combined with the high sensitivity of AFM single-molecule force spectroscopy allows us to investigate, quantify, and control molecular recognition processes (molecular nanotechnology), offering a tremendous potential in chemical biology.Single-molecule force spectroscopy experiments are able to detect fast intermediate transition states, details of ...
متن کاملVisualizing helicases unwinding DNA at the single molecule level
DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes move processively along DNA. Here, an optical method has been developed to follow the unwinding of multiple DNA molecules simultaneously in rea...
متن کاملStudying Self-Entangled DNA at the Single Molecule Level by
Knots seem to be found every time one encounters long, stringy objects. At the macroscopic scale, knots are seen every day in shoelaces, tangled hair, or woven clothing, yet they also present themselves at the microscopic scale in long polymer molecules. Knots can be found often in DNA packaged within the viral capsid, occasionally in proteins, and during the transcription and replication of ge...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Communications
سال: 2019
ISSN: 2041-1723
DOI: 10.1038/s41467-018-07924-1